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Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk betweenIgG sialylation and pDC functions

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 549-561 doi: 10.1007/s11684-022-0965-7

Abstract: Immunoglobulin (IgG) glycosylation affects the effector functions of IgG in a myriad of biological processesThis study aims to explore the relationship between IgG sialylation patterns and lupus pregnancy.- and deSia-IgG-treated pDCs.Finally, the coculture of pDCs isolated from pregnant patients with SLE with IgG/deSia-IgG demonstratedthe sialylation-dependent anti-inflammatory function of IgG.

Keywords： pregnancy     IgG glycome     type I interferon     systemic lupus erythematosus    

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Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Article

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Engineering 2023, Volume 26, Issue 7,   Pages 63-73 doi: 10.1016/j.eng.2022.09.012

Abstract: Profiling of the N-glycome in HD would assist in deciphering the underlying molecular mechanismN-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterizedThe serum level of IgG was found to serve as a good indicator for HD patients.It was notable that the changes in the galactosylation and sialylation of IgG1 and IgG2 were subclassInterestingly, altered sialylation and galactosylation of IgG2 or IgG3/4 strongly correlated with clinical

Keywords： Haff disease     Serum     Immunoglobulin G     Glycosylation     Disease pathogenesis    

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Frontiers of Medicine 2022, Volume 16, Issue 3,   Pages 378-388 doi: 10.1007/s11684-021-0840-y

Abstract: The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of

Keywords： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein    

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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 376-380 doi: 10.1007/s11705-015-1531-5

Abstract: synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilutionFinally, a high IgG1 production of 780 mg/L was obtained from a batch culture.

Keywords： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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The Glycome and Glycomedicine

Wei Wang, Baofeng Yang

Engineering 2023, Volume 26, Issue 7,   Pages 1-2 doi: 10.1016/j.eng.2023.05.007

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IgG N-Glycosylation Cardiovascular Age Tracks Cardiovascular Risk Beyond Calendar Age Article

Zhiyuan Wu, Zheng Guo, Yulu Zheng, Yutao Wang, Haiping Zhang, Huiying Pan, Zhiwei Li, Lois Balmer, Xia Li, Lixin Tao, Xiuhua Guo, Wei Wang

Engineering 2023, Volume 26, Issue 7,   Pages 99-107 doi: 10.1016/j.eng.2022.12.004

Abstract:

The use of an altered immunoglobulin G (IgG) N-glycan pattern as an inflammation metric hasHowever, the usable capacity of IgG N-glycosylation profiles for the risk stratification of cardiovascularThis study aimed to develop a cardiovascular aging index for tracking cardiovascular risk using IgG IgGdistance between GlyCage and calendar age independently indicates the cardiovascular risk, suggesting that IgG

Keywords： IgG N-glycosylation cardiovascular age     Cardiovascular aging     Immunoglobulin G     Glycosylation     Inflammation    

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Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infrared

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 386-406 doi: 10.1007/s11705-015-1533-3

Abstract: In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell

Keywords： technology (PAT)     Fourier-transform infrared (FTIR) spectroscopy     partial least squares (PLS) regression     mouse IgG3    

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Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus Article

Hudan Pan, Jingrong Wang, Yong Liang, Canjian Wang, Ruimin Tian, Hua Ye, Xiao Zhang, Yuanhao Wu, Miao Shao, Ruijun Zhang, Yao Xiao, Zhi Li, Guangfeng Zhang, Hua Zhou, Yilin Wang, Xiaoshuang Wang, Zhanguo Li, Wei Liu, Liang Liu

Engineering 2023, Volume 26, Issue 7,   Pages 89-98 doi: 10.1016/j.eng.2023.01.006

Abstract: Here, we identified two N-glycans on serum immunoglobulin G (IgG) as excellent diagnostic biomarkersOur findings provide insights into efficient population screening based on serum IgG glycosylation and

Keywords： Systemic lupus erythematosus     N-glycans     Diagnostic indicators    

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Twelve Years of Genome-Wide Association Studies of Human Protein N-Glycosylation Review

Anna Timoshchuk, Sodbo Sharapov, Yurii S. Aulchenko

Engineering 2023, Volume 26, Issue 7,   Pages 17-31 doi: 10.1016/j.eng.2023.03.013

Abstract: The lack of a detailed understanding of the mechanisms regulating glycome variation and linking the glycomeUntil now, such studies have been limited to an analysis of the human blood plasma N-glycome

Keywords： Glycome     Glycans     N-glycosylation     Genomics     Genetics     GWAS    

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Profound Diversity of the N-Glycome from Microdissected Regions of Colorectal Cancer, Stroma Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Engineering 2023, Volume 26, Issue 7,   Pages 32-43 doi: 10.1016/j.eng.2022.08.016

Abstract:

Aberrant glycosylation is considered to be a hallmark of colorectal cancer (CRC), as demonstrated by various studies. While the N-glycosylation of cell lines and serum has been widely examined, the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures. To overcome these obstacles, we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous, stromal, and healthy mucosa cells. N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray ionization tandem mass spectrometry, enabling the differentiation and structural characterization of isomeric species. In total, 116 N-glycans were identified that showed profound differences in expression among cancer, stroma, and normal mucosa. In comparison with healthy mucosa, the cancer cells showed an increase in α2-6 sialylation and monoantennary N-glycans, as well as a decrease in bisected N-glycans. Moreover, specific sialylated and (sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers. In comparison with cancer, the stroma showed lower levels of oligomannosidic and monoantennary N-glycans, LewisA/X epitopes, and sulfation, as well as increased expression of (core-)fucosylation and α2-3 sialylation. Our study reveals the distinct N-glycomic profiles of different cell types in CRC tumor and control tissues, proving the necessity of their separate analysis for the discovery of cancer-associated glycans.

 

Keywords： Colorectal cancer     Tumor     Porous graphitized carbon liquid chromatography mass spectrometry     N-glycomics     Antibody response    

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High-Throughput Profiling of Serological Immunoglobulin G N-Glycome as a Noninvasive Biomarker Article

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Engineering 2023, Volume 26, Issue 7,   Pages 44-53 doi: 10.1016/j.eng.2023.02.008

Abstract:

Immunoglobulin G (IgG) N-glycosylation plays a crucial role in the developmentThis study aimed to evaluate the diagnostic performance of IgG for gastrointestinal (GI) cancer subtypesN-glycome.Canonical correlation analysis (CCA) was used to explore the correlation between IgG N-glycosylationOur findings demonstrate that IgG N-glycosylation plays an important role in modulating the pathogenesis

Keywords： Gastrointestinal cancer     Glycosylation     Immunoglobulin G     Diagnostic biomarker    

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Title Author Date Type Operation

Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk betweenIgG sialylation and pDC functions

Journal Article

Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Journal Article

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Journal Article

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Journal Article

The Glycome and Glycomedicine

Wei Wang, Baofeng Yang

Journal Article

IgG N-Glycosylation Cardiovascular Age Tracks Cardiovascular Risk Beyond Calendar Age

Zhiyuan Wu, Zheng Guo, Yulu Zheng, Yutao Wang, Haiping Zhang, Huiying Pan, Zhiwei Li, Lois Balmer, Xia Li, Lixin Tao, Xiuhua Guo, Wei Wang

Journal Article

Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infrared

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

Journal Article

Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus

Hudan Pan, Jingrong Wang, Yong Liang, Canjian Wang, Ruimin Tian, Hua Ye, Xiao Zhang, Yuanhao Wu, Miao Shao, Ruijun Zhang, Yao Xiao, Zhi Li, Guangfeng Zhang, Hua Zhou, Yilin Wang, Xiaoshuang Wang, Zhanguo Li, Wei Liu, Liang Liu

Journal Article

Twelve Years of Genome-Wide Association Studies of Human Protein N-Glycosylation

Anna Timoshchuk, Sodbo Sharapov, Yurii S. Aulchenko

Journal Article

Pan Hudan: Changes in IgG Sugar Chain Structure in Rheumatic Disease and Its Clinical Application Value

29 Jun 2023

Conference Videos

Profound Diversity of the N-Glycome from Microdissected Regions of Colorectal Cancer, Stroma

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Journal Article

High-Throughput Profiling of Serological Immunoglobulin G N-Glycome as a Noninvasive Biomarker

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Journal Article